FIG. 7.

The ExoU-SOD1 interaction is maintained for C-terminal linker insertion molecules. An ELISA was developed to study ExoU-SOD1 binding. Recombinant protein binding, wash conditions, and controls for nonspecific interactions are described in Materials and Methods. (A) Control histidine-tagged proteins tested include WT rExoU (•), rD344A (▴), and rPcrV (♦). (B) Binding of 1.25 nM recombinant protein to bSOD1, with cumulative background control wells subtracted. The data are plotted as the average of 3 independent experiments, with error bars for standard error.