TABLE 2.

Screen summary for cytotoxic ExoU-LINKER molecules with supernatant phospholipase activity

Designation	Sequence	Membrane retention (OD570)
Vector	NA	1.3
WT ExoU	NA	0.275
S5	LVFKQ	0.245
S22	QVFKQ	0.262
P56	EMFKQ	0.275
Q68	GCLNK	0.222
I135	RCLNI	0.324
L173	LFKHL	0.476
L194	MFKQL	0.243
K195	CLNMK	0.336
G263	CLNTG	0.245
F290	KQAMF	0.315
E430	LFKHE	0.529
Q498	LFKHQ	0.100
G530	CLNNG	0.248
R581	VFKQR	0.192
V585	QSLFK	0.283
S587	LFKQS	0.381
Y619	LFKQY	0.139
W681	CLNTW	0.204

NA, not applicable.