FIG. 3.

Analysis of Mtz-induced killing and mutation over time. (A and B) Mtz^s wild-type C. jejuni 81-176 or 81-176 Sm^r cjj0275::cat-rpsL (LKB635) was grown and then not exposed (triangles) or exposed (circles) to 10 μg ml⁻¹ Mtz over the course of the assay. At each time point, bacteria were plated on MH agar containing TMP (closed symbols, solid lines or 10 μg ml⁻¹ Mtz (open symbols, dashed lines)) (A) or MH agar containing TMP (closed symbols, solid lines) or 2 mg ml⁻¹ Sm (open symbols, dashed lines) (B). The average number of viable bacteria at each time point is reported as the number of CFU per ml. Each assay was performed in triplicate, and the standard deviation is indicated by bars.