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. 2010 Jan 29;192(7):1890–1901. doi: 10.1128/JB.01638-09

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Copyright © 2010, American Society for Microbiology

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FIG. 6. — rdxA as a locus for genomic complementation of mutants in C. jejuni in cis. (A) Immunoblot analysis of FlhB production in wild-type, mutant, and complemented strains of C. jejuni. Total membranes from wild-type C. jejuni strains and respective mutant or complemented derivatives were analyzed for production of FlhB. Strains (from left to right) include DRH212 (wild-type 81-176 Sm^r), SNJ471 (81-176 Sm^r ΔflhB), DRH3067 (81-176 Sm^r ΔflhB rdxA::cat), DRH3107 (81-176 Sm^r ΔflhB rdxA::flhB), DRH533 (wild-type 81-176 Sm^r ΔastA flgDE2::nemo), DRH1827 (81-176 Sm^r ΔastA ΔflhB flgDE2::nemo), DRH3056 (81-176 Sm^r ΔastA ΔflhB flgDE2::nemo rdxA::cat), and DRH3057 (81-176 Sm^r ΔastA ΔflhB flgDE2::nemo rdxA::flhB). (B) Flagellar motility phenotypes of wild-type C. jejuni 81-176 Sm^r (DRH212) derivatives. Motility was assessed 30 h after inoculation in MH motility agar and incubation at 37°C under microaerobic conditions. Strains include DRH212, SNJ471, DRH3067, and DRH3107. (C) Arylsulfatase assays for analysis of expression of flgDE2::nemo, encoding an flgDE2-astA transcriptional fusion in C. jejuni 81-176 Sm^r ΔastA flgDE2::nemo derivatives. The results are from a typical assay with each strain tested in triplicate. The values reported for each strain are the average arylsulfatase activity ± standard deviation. Strains include DRH533, DRH1827, DRH3056, and DRH3057.