TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| M. xanthus | ||
| DK1622 | Wild-type strain | 15; D. Kaiser, University of Stanford |
| YL0301 | DK1622 ΔMXAN_4895 (groEL1 deletion) | This study |
| YL0302 | DK1622 ΔMXAN_4467 (groEL2 deletion) | This study |
| YL0305 | DK1622::pZC4895 (lacZ fused to groEL1) | This study |
| YL0306 | DK1622::pZC4467 (lacZ fused to groEL2) | This study |
| YL0307 | YL0301::pSWU4895 (groEL1 integrated at attB site) | This study |
| YL0308 | YL0307 ΔMXAN_4467 (groEL2 deletion) | This study |
| YL0309 | YL0302::pSW4467 (groEL2 integrated at attB site) | This study |
| YL0310 | YL0309 ΔMXAN_4895 (groEL1 deletion) | This study |
| E. coli | ||
| DH5α(λpir) | φ80 lacZΔM15 ΔlacU169 recA1 endA1 hsdR17 supE44 thi-1 gyrA relA1 λpir | H. B. Kaplan, University of Texas |
| XL1-Blue MR | Δ(mcrA)183Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac | Stratagene |
| Plasmids | ||
| pBJ113 | Gene replacement vector with KG cassette, Kmr | 14; Z. M. Yang, Virginia Tech |
| pMiniHimar1-lacZ | KmrlacZ | H.B. Kaplan, University of Texas |
| pSWU30 | Site-specific integration vector with Mx8 attP integration site, Tetr | Mignot Tâm, CNRS (Centre national de la recherche scientifique) |
| pSL1180 | Cloning vector, Ampr | Amersham |
| pSL1180-Km | 1.25-kb fragment of kan from pColE1 inserted into SmaI of pSL1180, Ampr Kmr | This study |
| pSW4467 | 2.14-kb fragment of MXAN_4467, with its upstream 495-bp promoter sequence, inserted into XbaI/EcoRI sites of pSWU30, Tetr | This study |
| pSW4895 | 2.93-kb fragment of MXAN_4895, with its upstream 1.28-kb promoter sequence, inserted into XbaI/EcoRI sites of pSWU30, Tetr | This study |
| pZCY11 | 1.56-kb HindIII/EcoRI fragment from pSL1180-Km, blunted with Klenow fragment and ligated with 4.57-kb T4 polymerase-blunted KpnI fragment from pMiniHimar1-lacZ, KmrlacZ | This study |
| pBJ4467 | Upstream and downstream homologous arms of DK1622 MXAN_4467 inserted into SmaI site of pBJ113, Kmr | This study |
| pBJ4895 | Upstream and downstream homologous arms of DK1622 MXAN_4895 inserted into SmaI site of pBJ113, Kmr | This study |
| pZC4467 | 601-bp fragment of DK1622 MXAN_4467, containing a stop codon, inserted into SpeI/KpnI sites of pZCY11, KmrlacZ | This study |
| pZC4895 | 685-bp fragment of DK1622 MXAN_4895, containing a stop codon, inserted into SpeI/KpnI sites of pZCY11, KmrlacZ | This study |