Strains |
|
|
M. xanthus
|
|
|
DK1622 |
Wild-type strain |
15; D. Kaiser, University of Stanford |
YL0301 |
DK1622 ΔMXAN_4895 (groEL1 deletion) |
This study |
YL0302 |
DK1622 ΔMXAN_4467 (groEL2 deletion) |
This study |
YL0305 |
DK1622::pZC4895 (lacZ fused to groEL1) |
This study |
YL0306 |
DK1622::pZC4467 (lacZ fused to groEL2) |
This study |
YL0307 |
YL0301::pSWU4895 (groEL1 integrated at attB site) |
This study |
YL0308 |
YL0307 ΔMXAN_4467 (groEL2 deletion) |
This study |
YL0309 |
YL0302::pSW4467 (groEL2 integrated at attB site) |
This study |
YL0310 |
YL0309 ΔMXAN_4895 (groEL1 deletion) |
This study |
E. coli
|
|
|
DH5α(λpir) |
φ80 lacZΔM15 ΔlacU169 recA1 endA1 hsdR17 supE44 thi-1 gyrA relA1 λpir
|
H. B. Kaplan, University of Texas |
XL1-Blue MR |
Δ(mcrA)183Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac
|
Stratagene |
Plasmids |
|
|
pBJ113 |
Gene replacement vector with KG cassette, Kmr
|
14; Z. M. Yang, Virginia Tech |
pMiniHimar1-lacZ
|
KmrlacZ
|
H.B. Kaplan, University of Texas |
pSWU30 |
Site-specific integration vector with Mx8 attP integration site, Tetr
|
Mignot Tâm, CNRS (Centre national de la recherche scientifique) |
pSL1180 |
Cloning vector, Ampr
|
Amersham |
pSL1180-Km |
1.25-kb fragment of kan from pColE1 inserted into SmaI of pSL1180, Ampr Kmr
|
This study |
pSW4467 |
2.14-kb fragment of MXAN_4467, with its upstream 495-bp promoter sequence, inserted into XbaI/EcoRI sites of pSWU30, Tetr
|
This study |
pSW4895 |
2.93-kb fragment of MXAN_4895, with its upstream 1.28-kb promoter sequence, inserted into XbaI/EcoRI sites of pSWU30, Tetr
|
This study |
pZCY11 |
1.56-kb HindIII/EcoRI fragment from pSL1180-Km, blunted with Klenow fragment and ligated with 4.57-kb T4 polymerase-blunted KpnI fragment from pMiniHimar1-lacZ, KmrlacZ
|
This study |
pBJ4467 |
Upstream and downstream homologous arms of DK1622 MXAN_4467 inserted into SmaI site of pBJ113, Kmr
|
This study |
pBJ4895 |
Upstream and downstream homologous arms of DK1622 MXAN_4895 inserted into SmaI site of pBJ113, Kmr
|
This study |
pZC4467 |
601-bp fragment of DK1622 MXAN_4467, containing a stop codon, inserted into SpeI/KpnI sites of pZCY11, KmrlacZ
|
This study |
pZC4895 |
685-bp fragment of DK1622 MXAN_4895, containing a stop codon, inserted into SpeI/KpnI sites of pZCY11, KmrlacZ
|
This study |