FIG. 3.
Luciferase activity (denoted in RLU/106 bacteria) detected in B. burgdorferi strains transformed with various PdbpBA-luc constructs. Spirochetes were cultured in BSK-H medium at 37°C and harvested at late log phase. Results from three independent experiments are presented as the mean values ± standard errors of the means (SEM). pOY63, cloning vector containing a promoterless luc; pOY64, vector containing WT PdbpBA; pOY66, vector containing ΔIR1 PdbpBA; pOY67, vector containing ΔIR1/2 PdbpBA; pOY69, vector containing minimal (Min) PdbpBA; pOY70, vector containing Δ−35 PdbpBA; and pOY71, vector containing Δ−35/−10 PdbpBA.