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Influence of temperature, pH (A and B), and whole blood (C) on luciferase expression driven by the B. burgdorferi ospC promoter (PospC) or flaB promoter (PflaB). Borrelia organisms were cultivated in BSK-H medium and harvested when growth reached a cell density of ∼1 × 106 to 5 × 106 spirochetes/ml. The experiments were replicated thrice, and bars represent the mean measurements ± SEM. The asterisk indicates statistical significance using Student's t test (P < 0.05).
