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Luciferase expressed from B. burgdorferi (A) or E. coli (B) containing various PdbpBA-luc constructs. Borrelia spirochetes were grown in BSK-H medium and collected at late log phase, whereas E. coli was grown in LB and harvested when growth reached an optical density at 600 nm of ∼0.6. Results from three tests are indicated as means ± SEM. The asterisk indicates statistical significance using Student's t test (P < 0.05).
