TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| B. burgdorferi | ||
| 297 | Infectious, low-passage B. burgdorferi | 27 |
| BbAH206 | 297, rpoS mutant | 27 |
| BbKH500 | 297, dbpBA mutant, Kanr | 3 |
| OY20 | 297 transformed with pOY63, Strr | This study |
| OY16 | 297 transformed with pOY64, Strr | This study |
| OY17 | 297 transformed with pOY66, Strr | This study |
| OY21 | 297 transformed with pOY67, Strr | This study |
| OY22 | 297 transformed with pOY68, Strr | This study |
| OY23 | 297 transformed with pOY69, Strr | This study |
| OY24 | 297 transformed with pOY70, Strr | This study |
| OY25 | 297 transformed with pOY71, Strr | This study |
| OY26 | 297 transformed with pOY72, Strr | This study |
| OY27 | 297 transformed with pJSB165, Strr | This study |
| OY28 | 297 transformed with pJSB175, Strr | This study |
| OY45 | BbKH500 transformed with pOY94, Kanr, Strr | This study |
| OY48 | BbKH500 transformed with pOY98, Kanr, Strr | This study |
| OY49 | 297 transformed with pOY109, Strr | This study |
| OY50 | BbKH500 transformed with pOY107, Kanr, Strr | This study |
| OY51 | BbKH500 transformed with pOY108, Kanr, Strr | This study |
| OY52 | BbAH206 transformed with pOY69, Strr | This study |
| E. coli | ||
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| Plasmids | ||
| pJD7 | B. burgdorferi/E. coli shuttle vector with PflgB-aadA; Spcr, Strr | 4 |
| pJD44 | B. burgdorferi/E. coli shuttle vector with aph[3′]-IIIa; Kanr | 41 |
| pJD48 | pJD44::promoterless lucBb+, Kanr | 4 |
| pJD54 | B. burgdorferi/E. coli shuttle vector with PflgB-aadA; Spcr, Strr | 4, 41 |
| pOY63 | promoterless lucBb+ from pJD48 cloned into pJD54 at BglII and HindIII; Spcr, Strr | This study |
| pOY64 | pOY63::WT PdbpBA (PCR product from primers ZM61 and ZM68); Spcr, Strr | This study |
| pOY66 | pOY63::ΔIR1 PdbpBA (PCR product from primers ZM61 and ZM54); Spcr, Strr | This study |
| pOY67 | pOY63::ΔIR1/2 PdbpBA (PCR product from primers ZM61 and ZM55); Spcr, Strr | This study |
| pOY68 | pOY63::PCR product from primers ZM61 and ZM56; Spcr, Strr | This study |
| pOY69 | pOY63::Min PdbpBA (PCR product from primers ZM61 and ZM57); Spcr, Strr | This study |
| pOY70 | pOY63::Δ−35 PdbpBA (PCR product from primers ZM61 and ZM58); Spcr, Strr | This study |
| pOY71 | pOY63::Δ−35/−10 PdbpBA (PCR product from primers ZM61 and ZM59); Spcr, Strr | This study |
| pOY72 | pOY63::PCR product from primers ZM61 and ZM60; Spcr, Strr | This study |
| pOY94 | pOY63::Min PdbpBA-dbpBA (PCR product from primers ZM102 and ZM104.3); Spcr, Strr | This study |
| pOY98 | pOY63::Δ−35 PdbpBA-dbpBA (PCR product from primers ZM103 and ZM104.4); Spcr, Strr | This study |
| pOY107 | pJD7::WT PdbpBA-dbpBA (PCR product from primers ZM109F and ZM110R); Spcr, Strr | This study |
| pOY108 | pJD7::ΔIR1 PdbpBA-dbpBA (PCR product from primers ZM110F and ZM110R); Spcr, Strr | This study |
| pOY109 | pOY63::Min PdbpBA with −14 C/A (PCR product from primers ZM61 and ZM57.2); Spcr, Strr | This study |
| pJSB165 | pJD7::divergently oriented PospC-Bbluc+; Spcr, Strr | 4 |
| pJSB175 | pJD7::divergently oriented PflaB-Bbluc+; Spcr, Strr | 4 |