TABLE 2.
Oligonucleotide primers used in this studya
| Name | 5′-3′ |
|---|---|
| ZM54 | CCATGGCCTTTTAAGCCTGCCAATCC |
| ZM55 | ATTTTAGATCTTTGATTCAATTTGC |
| ZM56 | AGATCTTTGTAATTCCAAACAATGTTACTGC |
| ZM57 | AGATCTATTTTATTTTATTTTTCATAAAGTGGGCTAAA |
| ZM57.2 | TATCCATGGATTTTATTTTATTTTTCATAAAGTGGGATAAAATTTAAATTTAAC |
| ZM58 | AGATCTCATAAAGTGGGCTAAAATTTAAAT |
| ZM59 | AGATCTATGAAAATTGGAAAGCTAAATTCAA |
| ZM60 | GGCAGATCTACATCAACATACTAACTA |
| ZM61 | CATATGCTTTTCCCGTGGCTTCTTTT |
| ZM68 | TATCCATGGTGCTTTCTTCTGCCAGGTC |
| ZM102 | TATCCATGGATTTTATTTTATTTTTCATAAAGTGGGCTAAA |
| ZM103 | TATCCATGGCATAAAGTGGGCTAAAATTTAAAT |
| ZM104.3 | GCCCATATGCTTTGGGTTAATTGCTTTAAC |
| ZM104.4 | TATCATATGTTTAGATTCTAAAGTTTAGATAAAAATTGGTCGGG |
| ZM109F | TAATGGCGCGCCTGCTTTCTTCTGCCAGGTC |
| ZM110F | TAATGGCGCGCCTTTTAAGCCTGCCAATCC |
| ZM110R | TAATGGCGCGCCTTTAGATTCTAAAGTTTAGATAAAAATTGGTCGGG |
| bba25-F | TGGCTATGTTTGACTTAATGCTTGAG |
| bba25-R | GATTCCTCTAAAACACGGGCTTTT |
| bba24-F | GGGTAGTGGGGTATCAGAAAATC |
| bba24-R | GAGCTGTAGTTGGAGGATTCTC |
| flaB-F | ACTCTTAAAGTCCAAGACGCTTGAG |
| flaB-R | TTGGAATGCAGCCTGCAAA |
| aadA-F | TAAGGCTTGATGAAACAACGCGGC |
| aadA-R | CGTCGTGCACAACAATGGTGACTT |
a
Restriction enzymes sites are underlined. In ZM57.2, the A used to replace the −14 C in the dbpBA promoter is indicated in boldface.