TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Sequence (5′→3′)a |
|---|---|
| eltb-bamh1 | CGGGATCCCGGAATTCGGGATGAATTATGAATAAAG |
| eltABreverse | AACTGCAGAACCAATGCATTGGCTAGTTTCCATACTGATTG |
| ltbL25Esense | CGATAAATGACAAGATAGAATCATATACGGAATCG |
| ltbL25Eantisense | CGATTCCGTATATGATTCTATCTTGTCATTTATCG |
| ltbQ3Ksense | CACACGGAGCTCCTAAATCTATTACAGAACTATG |
| ltbQ3Kantisense | CATAGTTCTGTAATAGATTTAGGAGCTCCGTGTG |
| ltbQ3Asense | CACACGGAGCTCCTGCATCTATTACAGAACTATG |
| ltbQ3Aantisense | CATAGTTCTGTAATAGATGCAGGAGCTCCGTGTG |
| ltbE11Ksense | CAGAACTATGTTCGAAATATCACAACACAC |
| ltbE11Kantisense | GTGTGTTGTGATATTTCGAACATAGTTCTG |
| ltbE11Asense | CAGAACTATGTTCGGCATATCACAACACAC |
| ltbE11Aantisense | GTGTGTTGTGATATGCCGAACATAGTTCTG |
| CTB_Q3K_sense | GGCGCATGCAACCCCTAAAAATATTACTGATTTG |
| CTB_Q3K_antisense | CAAATCAGTAATATTTTTAGGGGTTGCATGCGCC |
| CTBE11Ksense | CTGATTTGTGTGCAAAATACCACAACACAC |
| CTBE11Kantisense | GTGTGTTGTGGTATTTTGCACACAATCAG |
| CTB_E11A_sense | CTGATTTGTGTGCAGCATACCACAACACAC |
| CTB_E11A_anti | GTGTGTTGTGGTATGCTGCACACAAATCAG |
a
Restriction sites are italic; mutagenic codons are bold.