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. 2010 Jan 22;192(7):1882–1889. doi: 10.1128/JB.01503-09

FIG. 1.

FIG. 1.

Fractionation of proteins interacting with RNAPα by one-dimensional SDS-PAGE. After a preclearing step, the bacterial extract was incubated with 6HisRNAPα linked to agarose beads, and the protein components specifically recognized by the bait were eluted in Laemmli buffer. Lane 1, molecular markers; lane 2, total bacterial extract; lane 3, unbound proteins not retained by the bait; lane 4, protein extract retained by the His-Select nickel affinity gel alone and eluted with Laemmli buffer (control); lane 5, proteins specifically retained by the bait. Control lane 4 and sample lane 5 were cut into 38 slices, as indicated by boxes, and each gel slice from lane 4 (samples 1 to 38) and from lane 5 (samples C1 to C38) was subjected to mass spectrometry analysis.

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