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. 2010 Jan 22;192(7):1882–1889. doi: 10.1128/JB.01503-09

TABLE 1.

Bacterial strains and plasmids

Strain or plasmid Description Source or reference
Strains
    E. coli K-12 Wild type DSMZ
    C41(DE3) Derived from BL21 [FompT hsdSB(rB mB) gal dcm (DE3)]; has at least one uncharacterized mutation that prevents cell death associated with expression of many toxic recombinant proteins 15
    R721 71/18 glp O-P434/p22lacZ 5
Plasmids
    pET22b(+) N-terminal pelB signal sequence plus an optional C-terminal His-tagged sequence; ampicillin resistant Novagen
    pET28a(+) N-terminal His tag-thrombin-T7 tag configuration plus optional C-terminal His tag sequence; kanamycin resistance Novagen
    pET22b-αpol pET22b(+) Δ(NdeI-XhoI) Ω(rpoA) This study
    pET22b-c-Myc pET22b(+) Δ(NdeI-BamHI) Ω(c-myc tag) This study
    pET22b-c-Myc-L2 pET22b-c-Myc Δ(BamHI-XhoI) Ω(rplB) This study
    pET22b-c-Myc-Thioredoxin pET22 b-c-Myc Δ(BamHI-XhoI) Ω(trxC) This study
    pET28a-αpol pET28a Δ(BamHI-XhoI) Ω(rpoA) This study
    pcIP22 pC132 derivative carrying N-terminal end of p22 repressor; ampicillin resistance 5
    pcI434 pACYC177 derivative carrying N-terminal end of 434 repressor; chloramphenicol resistance 5
    pcIP22-RNAPα pcIP22Δ(SalI-BamHI) Ω(rpoA) This study
    pcI434-RNAPa pcI434Δ(SalI-BamHI) Ω(rpoA) This study
    pcI434-RPL2 pcI434Δ(SalI-BamHI) Ω(rplB) This study
    pcI-L2 pcI434 Δ(NdelI-BamHI) Ω(rplB) This study
    pcI-L1 pcI434Δ(NdelI-BamHI) Ω(rplA) This study
    pcI-L3 pcI434Δ(NdelI-BamHI) Ω(rplC) This study
    pcI-L20 pcI434Δ(NdelI-BamHI) Ω(rplT) This study
    pcI-L27 pcI434Δ(NdelI-BamHI) Ω(rpmA) This study
    pET22b-PrrnD pET22b Δ(SphI-HindIII) Ω(rrsD promoter) This study
    pET22b-PaidB pET22b Δ(SphI-HindIII) Ω(aidB promoter) This study
    pET22b-PrrnD-lacZ pET22b-PrrnDΔ(HindIII-XhoI) Ω(lacZ) This study
    pET22b-PaidB-lacZ pET22b-PaidBΔ(HindIII-XhoI) Ω(lacZ) This study
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