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. 2010 Jan 19;30(7):1673–1688. doi: 10.1128/MCB.00540-09

FIG. 3.

FIG. 3.

BC* degradation by the proteasome is Cul2 dependent. (A) Stability of BAF250b and BC* protein. HeLa cells transiently transfected with T7-BAF250bWT (lanes 1 to 3) or T7-BAF250bBC* (lanes 4 to 6) were treated with vehicle (−) or cycloheximide (CHX) for the times indicated. Whole-cell extracts were immunoblotted for T7-250b and endogenous BRG1. (B) BC* degradation is exacerbated by overexpression of Cul2. HeLa cells were transiently transfected with T7-BAF250b (lanes 1 to 6) or T7-BAF250bBC* (lanes 7 to 12) and Myc-Cul2 (lanes 1 to 12). Cells were treated with MG132 or CHX for the times indicated. Whole-cell extracts were immunoblotted for T7, Myc, or endogenous BRG1. (C) The degradation of BAF250bBC* is exacerbated by Cul2 but not Cul5. HeLa cells were transiently transfected with T7-BAF250bBC* (lanes 1 to 12) and Myc-Cul5 (lanes 1 to 6) or Myc-Cul2 (lanes 7 to 12). Cells were treated with MG132 or CHX for the times indicated. Whole-cell extracts were immunoblotted for T7, Myc, or endogenous Elo A (loading control).