FIG. 9.

Glypican-1 is present in the plasma membrane lipid rafts and in the extracellular matrix. (A) WT myoblasts were fractionated as shown in Fig. 7 after being washed with heparin in PBS Ca²⁺/Mg²⁺ (Heparin Wash) or PBS Ca²⁺/Mg²⁺ alone (control). The fractions were analyzed for HSPG core proteins and Cav-1 distribution. (B) Indirect immunofluorescence for glypican-1 (red) of cells treated as described for panel A. (C) Indirect immunofluorescence for glypican-1 (red) and laminin (green) in WT myoblasts.