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. 2010 Feb 1;30(7):1718–1728. doi: 10.1128/MCB.01301-09

FIG. 5.

FIG. 5.

In vitro effects of splicing modulators. (A) Constitutive splicing of CDC14-15 pre-mRNA was analyzed in the presence of 50 μM clotrimazole, flunarizine, or chlorhexidine for 90 min. Splicing intermediates are depicted to the left of the gel, and molecular size markers are indicated to the right of the gel. Fully spliced mRNA is indicated with a red arrow and corresponds to 140 nucleotides. (B) SR protein-regulated splicing of HIV Tat2-3, HβΔ6, and μC3-C4 pre-mRNAs was analyzed in the presence of DMSO or 50 and 100 μM chlorhexidine in vitro. Splicing intermediates are depicted to the left of the gels, and molecular size markers are indicated to the right of the gels. Red arrows point to the altered levels of the spliced mRNA, which are 371, 367, and 271 nucleotides for Tat2-3, HβΔ6, and μC3-C4, respectively.