FIG. 8.

Expression of MyoX in HUVECs and HeLa cells. (A) Detection of endogeneous MyoX by Western blotting. HUVEC and HeLa cell lysates were analyzed by Western blotting using the anti-MyoX pAb antibody. In HUVECs as well in HeLa cells, the antibody detected a 240-kDa band corresponding to the expected size of MyoX and several bands of lower molecular mass corresponding to MyoX degradation (6). Larger amounts of MyoX were detected in HeLa cells compared to HUVECs. (B) Partial colocalization of endogenous MyoX and VE-Cad along filopodia in subconfluent HUVECs. Subconfluent HUVECs were double labeled for MyoX (left) and VE-Cad (center) with anti-MyoX pAb, anti-VE-Cad MAb BV9, and Alexa 555-labeled goat anti-rabbit and Alexa 488-labeled goat anti-mouse antibodies and Hoechst stain. The merged image is at the right. Arrows point out VE-Cad patches colocalized with MyoX, while arrowheads indicate cell-cell junctions where VE-Cad and MyoX did not colocalize. Bar, 20 μm.