FIG. 6.

The C-terminal domain of BicD1 is involved in its trafficking to the viral assembly compartment. HFFs were transfected with truncated BicD1-GFP constructs and 24 h later were infected with HCMV. Five days later they were fixed and stained with anti-pp150 (red)- or anti-gM (blue)-specific MAbs and TRITC-conjugated goat anti-mouse IgG2b and Alexafluor-647 conjugated goat anti-mouse IgG1, respectively. (A) BicD1 aa 1 to 276-GFP (green), pp150 (red) and gM (blue). (B) BicD1 aa 630 to 820-GFP (green), pp150 (red), and gM (blue). (C) BicD1 aa 343 to 482-GFP (green), pp150 (red), and gM (blue). (D) BicD1 aa 1 to 482-GFP (green), pp150 (red), and gM (blue). (E) BicD1 aa 343 to 820-GFP (green), pp150 (red), and gM (blue). All images are at a magnification of ×600.