TABLE 1.

PCR primers/oligonucleotides used in this study

Primer or oligonucleotide	Sequence (5′-3′)	mer
Primers
Helicase forward	CATCCATATGGGCTGTCGGGTTACCCCCGG	30
Helicase reverse	CATCGGATCCTCAGAAAAAGTTATTAACGATTG	33
SM GKS forward	GGT GTG CCC GGG TCC GGC GCG TCC CGC TCT ATC ACC	36
SM GKS reverse	GGT GAT AGA GCG GGA CGC GCC GGA CCC GGG CAC ACC	36
SM DE forward	GGG CGC CGG GTT GTC ATT GCT GCG GCT CCA TCC CTC	36
SM DE reverse	GAG GGA TGG AGC CGC AGC AAT GAC AAC CCG GCG CCC
RNA oligonucleotides
RNA1	UUU UUU UUU UUU CGC UGA UGU CGC CUG G	28
RNA2	CCA GGC GAC AUC AGC G	16
RNA3	CGC UGA UGU CGC CUG GUU UUU UUU UUU U	28
RNA4	CCA GGC GAC AUC AGC GAA AAA AAA AAA A	28
Poly(A)	AAA AAA AAA AAA AAA AAA A	19
Poly(G)	GGG GGG GGG GGG GGG GGG G	19
Poly(C)	CCC CCC CCC CCC CCC CCC C	19
Poly(U)	UUU UUU UUU UUU UUU UUU U	19
DNA oligonucleotides
DNA1	TTT TTT TTT TTT CGC TGA TGT CGC CTG G	28
DNA2	CCA GGC GAC ATC AGC G	16
DNA3	CGC TGA TGT CGC CTG GTT TTT TTT TTT T	28
DNA4	CCA GGC GAC ATC AGC GAA AAA AAA AAA A	28
Poly(A)	AAA AAA AAA AAA AAA AAA A	19
Poly(G)	GGG GGG GGG GGG GGG GGG G	19
Poly(C)	CCC CCC CCC CCC CCC CCC C	19
Poly(T)	TTT TTT TTT TTT TTT TTT T	19