FIG. 4.

CAV9 is not internalized into A549 cells in association with αVβ6 integrin. A549 cells were grown on coverslips for 24 h and infected with CAV9 at 60% efficiency of infection. Unbound virus was removed, warm medium was added (0 min), and cells were transferred to 37°C. The cells were incubated for 5 or 20 min before they were fixed and permeabilized. Cells were stained with virus-specific polyclonal antiserum and AF 488-labeled secondary antibody (green) and with integrin αvβ6-specific monoclonal antibody and AF 568-labeled secondary antibody (red) prior to confocal imaging. Bar, 10 μm.