FIG. 8.

β2M is required for CAV9 infection. A549 cells were transfected with control siRNA or β2M siRNA (Hs_β2M_3) and cultured for 48 h. The cells were infected with CAV9 at 60% efficiency of infection, incubated on ice for 1 h, and washed. Warm medium was added at the 0-min time point; the cells were transferred to 37°C and incubated for 5 min, 20 min, and 6 h before fixing, permeabilization (except for the 0-min sample), staining with CAV9- and β2M-specific antibodies, and confocal imaging. CAV9 is shown in green, β2M is in red, and nuclei are in blue. A silenced cell in the 0-min images is indicated by an arrow. Bar, 10 μm.