FIG. 3.

HvAV-3e RNase III cleaves dsRNA. (A) Expression of RNase III protein in Sf9 cells infected with RNase III recombinant baculovirus. Samples were collected at 48 and 72 hpi and analyzed by Western blotting with an antiserum against the His tag. Lane M, mock infection. An ∼80-kDa fusion protein is indicated by an arrow. (B) In vitro catalysis of Bro11 and GFP dsRNAs in the presence of recombinant RNase III. No enzyme and the Vn50 protein were used as controls. (C) In vivo catalysis of GFP dsRNA in Sf9 cells. At 48 h after the transfection of 0.5 μg of dsRNA, Sf9 cells were inoculated with Vn50 baculovirus, mock inoculated, inoculated with HvAV-3e, and inoculated with RNase III recombinant baculovirus (lanes 1, 2, 3, and 4, respectively). rRNA bands show equal loading of the total RNA samples in the gel.