FIG. 6.

RNase III is required for HvAV-3e pathology and DNA replication. (A) Phase-contrast images of HzFB cells at 48 hpi after mock transfection (subpanel 1) or transfection with RNase III (subpanel 2) and GFP dsRNA (subpanel 3). Subpanel 4 shows mock-transfected cells that were mock infected. (B) Northern hybridization showing downregulation of RNase III transcript levels in RNase III-specific dsRNA transfected cells after 48 h of virus infection. (C) qPCR analysis of total genomic DNA from cells treated as in panel A that shows the relative viral DNA levels in the treatments. Error bars represent the standard deviations of averages from three replicates.