FIG. 6.
Intracellular colocalization of Lassa virus proteins GP, Z, and NP. Huh 7 cells transiently expressed LASV glycoprotein GPHAtag, NP, and/or Z. Cells were methanol-acetone fixed at 24 h after transfection, and LASV proteins were detected using chicken α-HA tag (green)-, mouse α-m-NP (red)-, and rabbit α-Z (blue)-specific antibodies. The immunofluorescence microscopic analyses were done by sequential scanning using confocal microscopy. Single expression of GP, NP, and Z (A to C); double expression of GP and Z (D to H), NP and Z (I to M), or GP and NP (N to R); and triple expression of GP, NP, and Z (S to W) are shown. Enlarged sections of merged images (H, M, R, and W) that contain fluorescence profile lines are additionally shown. Arrows indicate colocalization.