FIG. 2.
Cell-free and cell-cell infections are equally susceptible to entry inhibition. (A) A301.R5 target cells were either mixed 1:1 with JktBaL cells or with 12-h cell-free supernatants derived from JktBaL in the presence of titrations of the inhibitors shown. Cells were harvested after 12 h and processed for qPCR of pol and β-globin. Reduction in the pol PCR signal relative to the β-globin signal was expressed for each dilution of each inhibitor. The background signal obtained from nonpermissive A201 cells was subtracted from all of the values before calculation of the percent inhibition compared to no-treatment or no-virus controls. Solid lines represent cell-free virus inhibition, and dashed lines represent cell-cell virus inhibition. (B) Percentage inhibition data from three independent experiments each carried out in triplicate were used to calculate IC50s. □, Cell-free infection inhibition; ▪, cell-cell infection inhibition. Error bars represent the SEM.