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. 2010 Jan 27;84(7):3287–3302. doi: 10.1128/JVI.02020-09

FIG. 5.

FIG. 5.

The cellular environment regulates the interaction between Akt and M-T5. Cells were transiently transfected with the specified (+) combination of plasmids (MT5/myc-His, HA-Akt, HA-AktΔ2, and HA-Myr-Akt) in HEK293 (A) or Caki, 786-0, and MCF-7 (B and C) cell lines. Cell lysates were harvested after 48 h and preincubated with biotin conjugated anti-Myc antibody and donor/acceptor beads before an AlphaScreen assay was performed to determine binding. Each sample was assayed in triplicate, and the error bars represent standard deviations. (C) Cells were either mock treated or treated with Akt inhibitor VIII or X prior to collection of cell lysis and AlphaScreen analysis of M-T5 binding to Akt.