FIG. 10.
Growth properties of recombinant viruses in MeWo cells and MRC-5 cells. Cells were infected with rpOka, rpOkagMim, or rpOkagMimRev (log10 2.0 PFU/well), harvested at the indicated time points, serially diluted, added to newly prepared cells on 6-well plates, and cultured for 5 days. The plaques were stained with crystal violet and counted. Each point represents the mean titer for two wells of one experiment. The experiments were done twice independently. Statistical significance was determined by Student's t test.