TABLE 1.

Primers used for mutation of the ORF50 gene

Primer	Sequencea	Amino acid mutation
ORF50 124gt-cc	5′-ttg gat aat tca gCC gat gat gtt cac-3′	42 Valine to proline (V42P)
ORF50 125t-c	5′-ttg gat aat tca ggC gat gat gtt cac-3′	42 Valine to alanine (V42A)
ORF50 448t-c	5′-gct ccg gtg ttg Cat ggc tag ttg tag atc-3′
ORF50 900a-c	5′-att ata tac acg tgt taa tCg gac cgt ccc-3′
ORF50 900agga-catg	5′-att ata tac acg tgt taa tCA TGc cgt ccc-3′	301 Glycine to methionine (G301M)
ORF50 1101g-a	5′-gca tat gtg tat cat cga caA aaa cgc ag-3′

^aNucleotides that differ from those of wild-type ORF50 are shown in capital letters.