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. 2010 Jan 20;84(7):3586–3594. doi: 10.1128/JVI.01975-09

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FIG. 4. — Time course and efficacy of T_CD8⁺ activation after i.d. infection with AdCMVNP or AdK14NP. Mice were immunized i.d. and tested for either the ability to stimulate proliferation of adoptively transferred F5 T_CD8⁺ via CFDA-SE dilution (A and B) or the effector activity via clearance of specific peptide-pulsed CFDA-SE^HI labeled targets (C) on day 3 (A) or days 2, 3, and 4 (B and C) postinfection. (A) The site of infection was removed at the times shown postinfection. (D) The sensitivity of effector F5 T_CD8⁺ was assessed by measuring the clearance of cells pulsed with various amounts of NP_366-374 peptide 6 days after i.d. immunization. The data in panel C are expressed as a percentage of the maximal killing of NP_366-374 peptide-pulsed CFDA-SE^HI cells compared to levels after immunization with AdSPCNP. *, P < 0.05; **, P < 0.01.