FIG. 1.

Characterization of BacHA. (A) Indirect immunofluorescence assay with insect cells infected with recombinant baculovirus expressing H5 HA0. Infected cells were fixed and stained with guinea pig anti-HA antibody and rabbit anti-guinea pig FITC. (B) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of purified baculoviruses, showing the presence of major capsid protein (VP39) and envelope protein (GP64) of baculovirus and influenza HA in the purified sucrose gradient fraction. Lane 1, prestained protein marker; lane 2, infected cell culture supernatant; Lanes 3, 4, and 5, first, second, and third fractions from sucrose density gradient purification, respectively. (C) Western blot. The antigenic conformation of HA incorporated into purified baculoviruses (fraction 3) was probed using anti-HA monoclonal antibody.