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Measurement of systemic immune response. Groups of mice (n = 6) were orally vaccinated three times on days 0, 7, and 21 with 200 μl containing inactivated or live recombinant baculovirus at a log2 HA titer of 8, adjuvanted with or without 10 μg rCTB. (A) HA-specific IgG antibody titers in serum, determined by indirect ELISA. (B) Serum hemagglutination inhibition titer. Each point represents the arithmetic mean value (n = 6) ± SD (***, P < 0.001).
