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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: Int Rev Neurobiol. 2009;88:199–222. doi: 10.1016/S0074-7742(09)88008-2

FIG. 4.

FIG. 4

Effect of the DARPP-32-siRNA transfection on cell viability in NHA. Cell viability was measured using the MTT assay. NHA were transfected with DARPP-32-siRNA (200 pM) using siPROT transfection reagent from Ambion Inc. (Austin, TX), for a time period of up to 96 h. Data were expressed as a percentage of viable cells calculated with respect to the untreated control and represented as the mean ± S.D. of three separate experiments done in duplicate. Our results showed a >90% viability over the range of time periods tested.