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. Author manuscript; available in PMC: 2010 Mar 15.
Published in final edited form as: Int Rev Neurobiol. 2009;88:199–222. doi: 10.1016/S0074-7742(09)88008-2

FIG. 5.

FIG. 5

Effect of the DARPP-32-siRNA transfection on DARPP-32 gene silencing in NHA. NHA were transfected with DARPP-32-siRNA (200 pM) using siPROT transfection reagent from Ambion Inc. (Austin, TX), for a time period of up to 1 week. NHA were treated in vitro with the DARPP-32-siRNA (200 pM) and scrambled-siRNA (transfection control), RNA was extracted, reverse transcribed, cDNA amplified, and the DARPP-32 gene expression was determined by real time quantitative PCR. Relative expression of mRNA species was calculated using the comparative CT method. Data are the mean ± S.D. of three separate experiments done in duplicate. Statistical significance was determined using ANOVA based on comparison between, the positive control, DARPP-32 siRNA and the scrambled-siRNA samples. Our results show a >80% suppression in DARPP-32 gene expression in NHA that were transfected with DARPP-32 siRNA.