FGF-2 neutralization reduces TNFα endothelial cell apoptosis. Endothelial cells cultured in 5 or 30 mM glucose supplemented media were given fresh media or fresh media with 10 ng/ml FGF-2, 1 µg/ml FGF-2 neutralizing antibody, 5 ng/ml TNFα, TNFα with FGF-2, or TNFα with FGF-2 antibody. After (A,C) 12 h or (B,D) 24 h cells were harvested and prepared for annexin V—propidium iodide flow cytometry. Data shown is one representative experiment of three, n = 3. *P < 0.01.