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. 2010 Jan 22;285(12):8563–8571. doi: 10.1074/jbc.M109.062646

TABLE 1.

NGR and iso-DGR/DGR sequences detected by LTQ-MS/MS analysis of tryptic fragments of reduced and alkylated proteolytic N-terminal FN fragments

p, plasma; r, recombinant. *, alkylation of cysteine; ND, peptide not detected. Asn263 and Asn501 are underlined.

Attribute of peptide Tryptic peptide
253GNLLQC*IC*TGNGR
494C*TC*VGNGRGEWTC*IAYSQLR
Unmodified Modified Unmodified Modified
Expected mass 1462.69 1463.67 2388.69 2389.68
Found in p70K 1462.08a 1463.10a 2388.06 ND
Found in p40K ND ND 2388.06 ND
Found in r70K 1462.64 ND 2388.90 ND

a MS/MS shown in Fig. 1.