TABLE 1.
NGR and iso-DGR/DGR sequences detected by LTQ-MS/MS analysis of tryptic fragments of reduced and alkylated proteolytic N-terminal FN fragments
p, plasma; r, recombinant. *, alkylation of cysteine; ND, peptide not detected. Asn263 and Asn501 are underlined.
Attribute of peptide | Tryptic peptide |
|||
---|---|---|---|---|
253GNLLQC*IC*TGNGR |
494C*TC*VGNGRGEWTC*IAYSQLR |
|||
Unmodified | Modified | Unmodified | Modified | |
Expected mass | 1462.69 | 1463.67 | 2388.69 | 2389.68 |
Found in p70K | 1462.08a | 1463.10a | 2388.06 | ND |
Found in p40K | ND | ND | 2388.06 | ND |
Found in r70K | 1462.64 | ND | 2388.90 | ND |
a MS/MS shown in Fig. 1.