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In vivo complementation assay. E. coli CY1861 were transformed with pMAL-derived plasmids encoding the indicated proteins under control of the IPTG-inducible ptac promoter. Cells were grown in medium containing arabinose (open circles) or IPTG and glucose (closed circles), and the absorbance at 595 nm was recorded over 6 h. Growth curves representative of three individual experiments are shown.
