FIGURE 3.

BanLec inhibits HIV-1 infection of MDM. A, MDM were pretreated with BanLec for 30 min before the addition of 100 TCID₅₀ of HIV-1 NL(AD8). Twenty-four hours later, the media was removed, and the cells were washed with PBS to eliminate remaining virus. Fresh media containing BanLec or PBS was added to the cells. A sample of culture supernatant was taken every 3 days for p24 quantification by ELISA and replaced with new media containing lectin in PBS or PBS alone as a control. On day 15, viability was assessed by an MTT assay, which indicated no cellular toxicity (data not shown). B, MDM were pretreated and infected with HIV-1 as described above. 24 h post-infection the cells were washed with PBS to remove residual virus and cultured in media containing BanLec or PBS. Seven days post-infection, supernatants were removed for determination of p24 antigen as detected by ELISA. The concentration for a 50% reduction in p24 production was calculated to be 9.72 nm. Cellular viability was assessed by an MTT assay, and no toxicity was observed (data not shown). Results shown in panels A and B are representative of three and two separate experiments, respectively.