FIGURE 6.

Binding of gp120 by BanLec blocks access to the anti-HIV monoclonal antibody 2G12. Recombinant gp120-coated ELISA plates were treated with different concentrations of BanLec before incubation with the 2G12 antibody, which recognizes the high mannose structures found at positions Asn-295, -332, and -392 of the gp120 protein. Unbound antibody was removed, and the amount of antibody remaining was determined by comparison to a standard curve. The results shown represent the average from three separate experiments. Error bars represent S.E. The effect of increasing amounts of BanLec was determined to be significant by 1-way analysis of variance testing (p < 0.01).