FIGURE 3.

Cell surface EpCAM expression and isolation of hESCs. A, cell surface EpCAM protein expression by undifferentiated hESCs (H9, hES5, HUES3, and HUES6) and feeder MEF by ELISA using an anti-EpCAM mAb (**, p < 0.01). B, flow cytometry analysis of EpCAM on H9 hESCs co-cultured with MEF (top) and MEF alone (middle) and analysis of CD29 on MEF alone (bottom). C, analysis of cell surface expression of EpCAM on H9 cells co-cultured with MEF by fluorescent flow cytometry. D and E, double labeling of EpCAM positive (D) and EpCAM negative (E) population with anti-SSEA4 and anti-CD29 antibodies on undifferentiated H9 cells co-cultured with MEF.