Phenotypic changes after development of zebrafish eggs that had been injected with pT7vaRz(ntl)435, pT7T7, and T7 RNA polymerase. (A) Normal zebrafish at 96 h of embryogenesis. (B) Two examples of partial no tail phenotypic changes at 96 h of embryogenesis in zebrafish developing from eggs injected with pT7vaRz(ntl)435, pT7T7, and T7 RNA polymerase. (C) An example of full no tail phenotypic changes at 96 h of embryogenesis in zebrafish developing from eggs injected with pT7vaRz(ntl)435, pT7T7, and T7 RNA polymerase. (D) A homozygous no tail mutant zebrafish (ntlb195) (23) at 96 h of embryogenesis.