. 1997 Dec 9;94(25):13777–13781. doi: 10.1073/pnas.94.25.13777

Table 1.

Zebrafish egg injections and resulting phenotypic change

Injected material	Egg injected, n	Egg survived 96 h postfertilization, n	Phenotypic change
Injected material	Egg injected, n	Egg survived 96 h postfertilization, n	None	Partial	Full
Uninjected	—	501	501	0	0
pT7va^* + pT7T7 + T7 RNAP	508	211	211	0	0
pT7vaRz(ntl)435^†+ pT7T7 + T7 RNAP	799	316	256	38 (12%)^‡	22 (7%)^‡
pT7vaRz(ntl)435-11^† + pT7T7 + T7 RNAP	257	85	85	0	0
pT7vaRz(ntl)435-22^† + pT7T7 + T7 RNAP	426	135	135	0	0
pT7vaRz(ntl)435-S^† + pT7T7 + T7 RNAP	631	220	220	0	0

The data shown were the results of several individual injection experiments. Each injection resulted in quantitatively similar percentages of the phenotypic changes in the injected zebrafish embryos. See text for discussion. The phenotypic changes are as defined and shown in Fig. 2.

pT7va is a control plasmid produced by deletion of ribozyme coding sequences from pT7vaRz.

^†

The complementary flanking sequences (binding arms) are as follows:

Rz(ntl)435: 5′-CCTCCGTT.......AGTTTATT-3′

Rz(ntl)435-11: 5′-CCTCCCTT.......AGATTATT-3′

Rz(ntl)435-22:5′-CCACCCTT.......AGATTTTT-3′

Rz(ntl)435-S: 5′-CTCTACTT.......TTGCTGTA-3′

^‡

The percentage is calculated by dividing the number of the phenotypic change by the number of the survived.