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EPR spectra of the reduced wild-type and mutant enzymes. EPR conditions were as follows: temperature, 9 K; microwave power, 20 milliwatts at 9.381 GHz; and modulation amplitude (peak to peak), 20 Gpp at 100 KHz. Spectra are of membrane samples normalized to a protein concentration of ∼30 mg ml−1. WT, wild type.
