FIGURE 3.

A, RNA band shift performed with increasing amounts of GST-hnRNP A18 (0.05, 0.1, 0.25, and 0.5 μg) and the hnRNP A18 motif version 1 (lanes 1–5) and the hnRNP A18 motif in ATR 3′-UTR (nucleotides 150–199, lanes 6–10). Recombinant GST (0.5 μg) was used as a negative control, and tRNA (50 μg/ml) was used as a nonspecific competitor. B, same as A with the exception that the R1 and R2 probes were used. C, sequence and predicted structures of the R1 and R2 RNA probes.