TABLE 2.
Oligonucleotides
| Name | Oligonucleotide sequencea |
|---|---|
| TrwCNdeI | TCACTCATATGCTCAGTCACATGGTATTGACC |
| TrwC293END | GGGGGATCCTTAGCTGAAATCTATGCCG |
| oriT322EcoRI | GGCGAATTCGTAGTGTTACTGTAGTGG |
| oriTR388HindIII | TGCATCATTGAAGCTTGATAACCCAATG |
| R(12 + 18)mut26–27 | TGCGTATTGTCTCGAGCCCAGATTTAAGGA |
| R(12 + 18)mut28–29 | TGCGTATTGTCTATCTCCCAGATTTAAGGA |
| R(35 + 8)mutIR | AATGACTTACGGCGTGGGAAACCACGCTATTGTCTATAGCCCA |
| R388–33comp | TGGGCTATAGACAATACGCACCTTTCGGTGCGC |
| R46nic(31 + 8) | ATAGCGTGATTTATGCCGCTGCGTTAGGTGT↓ATAGCAGG |
| Fnic(29 + 10) | CAGCAAAAACTTGTTTTTGCGTGGGGTGT↓GGTGCTTTTG |
| R(35 + 8) | AATGACTTACGCGCACCGAAAGGTGCGTATTGTCT↓ATAGCCCA |
| R(25 + 8) | GCGCACCGAAAGGTGCGTATTGTCT↓ATAGCCCA |
| R(22 + 11) | CACCGAAAGGTGCGTATTGTCT↓ATAGCCCAGAT |
| R(19 + 14) | CGAAAGGTGCGTATTGTCT↓ATAGCCCAGATTTA |
| R(16 + 17) | AAGGTGCGTATTGTCT↓ATAGCCCAGATTTAAGG |
| R(12 + 18) | TGCGTATTGTCT↓ATAGCCCAGATTTAAGGA |
| R(14 + 4) | GGTGCGTATTGTCT↓ATAG |
| R(12 + 4) | TGCGTATTGTCT↓ATAG |
| R(6 + 4) | TTGTCT↓ATAG |
| R(25 + 4) | GCGCACCGAAAGGTGCGTATTGTCT↓ATAG |
| R(25 + 0) | GCGCACCGAAAGGTGCGTATTGTCT↓ |
| R(25-3) | GCGCACCGAAAGGTGCGTATTG |
| R(25-6) | GCGCACCGAAAGGTGCGTA |
| Rm(28-29) | GCGCACCGAAAGGTGCGTATTGTCT↓ATCTCCCA |
| Rm(26-27) | GCGCACCGAAAGGTGCGTATTGTCT↓CGAGCCCA |
| Rm(23-25) | GCGCACCGAAAGGTGCGTATTGGAG↓ATAGCCCA |
| Rm(20-22) | GCGCACCGAAAGGTGCGTAGGCTCT↓ATAGCCCA |
| Rm(18-19) | GCGCACCGAAAGGTGCGGCTTGTCT↓ATAGCCCA |
| R3m(17) | GCGCACCGAAAGGTGCCTATTGTCT↓ATAGCCCA |
| Rm(13-16) | GCGCACCGAAAGTGTAGTATTGTCT↓ATAGCCCA |
| Rm(8-11) | GCGCACCTCCCGGTGCGTATTGTCT↓ATAGCCCA |
| Rm(4-7) | GCGACAAGAAAGGTGCGTATTGTCT↓ATAGCCCA |
| Rm(IR) | GGCGTGGGAAACCACGCTATTGTCT↓ATAGCCCA |
a The sequence that corresponds to the inverted repeat IR2 of R388 nic is underlined. Nucleotides that are different from R388 wild type sequence are shown in boldface type. The downward arrow indicates the position of the nic cleavage site.