TABLE 2.

Effect of reconstitution of multiple P450 enzymes on monooxygenase function: Preformed DLPC vesicles

Reconstituted systems and mixtures of systems described in Table 1 were prepared in DLPC and used to measure the metabolism of the listed substrates as described under “Experimental Procedures.” The abbreviations used are the same as those indicated in Table 1. The values represent the mean (nmol/min) ± S.D. of triplicate determinations. ND, not detected.

Substrate	2B4/CPR	1A2/CPR	SUM	1A2/CPR/2B4 (change)
Luciferin-ME	ND	0.3 ± 0.1	0.3	0.6 ± 0.1 (+152%)
7ER	0.1 ± 0.0	0.2 ± 0.0	0.3	0.3 ± 0.0 (+17%)
7EFC	21.2 ± 1.5	0.5 ± 0.1	21.7	15.5 ± 0.6 (−30%)
Benzphetamine	10.2 ± 1.6	0.7 ± 0.2	10.9	8.4 ± 0.0 (−22%)
7EC	20.3 ± 0.7	0.3 ± 0.10	20.6	10.4 ± 1.4 (−49.5%)
p-Nitroanisole	1.4 ± 0.2	0.3 ± 0.1	1.7	0.9 ± 0.1 (−46%)