FIGURE 6.

Agonistic anti-DR5 mAbs and TRAIL induce DISC formation in Jurkat cells and H460 cells. A and B, analysis of TRAIL- and agonist anti-DR5 mAbs-induced DISC in Jurkat cells (A) and H460 cells (B). Cells were treated with anti-DR5 mAbs or “TRAIL-FLAG + anti-FLAG” for indicated time before cell lysis, although AD5-10 was added to the cells after cell lysis in unstimulated cells. The samples were pre-cleared with-Sepharose 4B (GE Healthcare) and immunoprecipitated (IP) overnight at 4 °C with protein G-Sepharose beads and analyzed by Western blotting. All experiments were repeated at least three times with similar results. IB, immunoblot; WCE, whole cell extract.