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. 2010 Jan 19;285(12):8967–8975. doi: 10.1074/jbc.M109.058107

FIGURE 6.

FIGURE 6.

Comparative Tau values for all PrP(128X) paired with PrP-128M and PrP-128V. A, FRET of homologous PrP(128X/X) pairs. The Tau for each homologous PrP(128X/X) pair (black bars) are represented relative to WT PrP(128M/M) (gray bar). The label below each bar indicates the amino acid substitution at residue 128. PrP(128X)::CFP donor was co-expressed with its homologous PrP(128X)::YFP acceptor, and FRET performed on 35 to 50 separate co-aggregates for each substitution. Each homologous pair resulted in reproducible and significant FRET over a non-FRET control (see Table 1). B, relative Tau of heterologous PrP(128X/V) pairs (black bars) compared with the homologous PrP(128V/V) pair (gray bar). PrP-128V::CFP was the donor and PrP(128X)::YFP the acceptor, and X is each substitution, as labeled. The non-FRET PrP(128V/YFP) is also represented (−). C, relative Tau of heterologous PrP(128X/M) pairs (black bars) relative to the homologous PrP(128M/M) pair (gray bar), with PrP-128M::CFP as donor and PrP(128X)::YFP as acceptor, and X is each substitution, as labeled. The non-FRET PrP(128M/YFP) pair is also represented (−). D, Tau values for PrP-128K paired with PrP-128K, PrP-128M, or PrP-128V, compared with the homologous PrP(128M/M) pair (black bars) and the non-FRET control (gray bars), as labeled. For all experiments in this figure, *, p < 0.05; **, p < 0.001. All values, except those in panel D, are also provided in Table 1. M, Met; A, Ala; L, Leu; I, Ile; Y, Tyr; V, Val; K, Lys.