TABLE 3.

ΔΔG(c-t) calculated from the percentage of cis results of solution NMR experiments for each amino acid and the ΔΔG(EC₅₀) calculated from electrophysiology results of mutant receptors containing the corresponding amino acid at α136

ΔΔG(c-t) = RTln(% cis(Pro analogue)/% cis(Pro)). ΔΔG(EC₅₀) = −RTln(EC₅₀(Pro analogue)/EC₅₀(Pro)).

XPro	Percentage cis	ΔΔG(c-t)	ΔΔG(EC50)
	%	kcal/mol	kcal/mol
Pro	17	0	0
Pip	39	0.65	1.5
Aze	30	0.42	0.81
Flp	32	0.49	0.38
3-Me-Pro	12	−0.24	1.8
2-Me-Pro	0		−0.056
Mor	48	0.87	0.57