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Constructs and purified proteins. A, shown is the domain architecture of different constructs used in the study. B, His-tagged proteins were purified from E. coli strain BL21 (DE3) using Ni-NTA affinity purification followed by fast protein liquid chromatography. Protein purity was estimated by Coomassie staining. The correct molecular size of G18ΔC (which may have run anomalously due to its high proline content) was verified by mass spectrometry.
