TABLE 1.

Kinetic parameters of TrmH for tRNA variants

Not detectable means that the methyl transfer could not be detected under the tested conditions.

Variant	Km	Vmax	Relative Vmax/Km
	nm	μmol/mg h
Wild type tRNAPhe transcript	82	3.1	1.0
tRNAPhe-dG18	820	0.11	0.0036
tRNAPhe-dG18dG19A20	Not detectable	Not detectable	Not detectable
tRNAPhe-GGA	245	1.2	0.13
tRNAPhe-GAA	200	0.135	0.018
tRNAPhe-AGA	12500	0.00675	0.000014
tRNAPhe-GUA	300	0.18	0.016